Quantitation Of Protein

Introduction In today’s world we estimate on pharmaceutical drugs more than ever and production of these drugs desire insulin or human growth hormone, require quantitation of protein. By playacting experiments on quantitation of protein we give the axe discover meteoric methods or new shipway to achieve this and as well as see which method is the most cost-effective. every(prenominal) company wants an profit over the former(a) companies, so if they stop find a faster or more efficient way of quantitating protein, they can excite more money comp bed to the other companies. This lab uses a surmisal of spectrophotometry and an understanding of the Beer-Lambert law; it also uses three hindrances utilise to quantitate protein. Spectrophotometry uses a device called a Spectrophotometer which can measure the absorbance of decipherable at different wavelengths in a resoluteness. The Beer-Lambert’s law relates the absorbance to the compactn ess of the solution, the pathlength of the cuvette and the extinction coefficient (A=ecl). Three stresss used to quantitate protein in this lab are Coomassie Blue, Lowry and UV direct. Coomassie blue is a dye binding method, where it binds to the protein state in the solution and changes the discolour of the solution which is then metric by the spectrophotometer.

Lowry assay is where the cupric ions react with the peptide bonds under alkalescent conditions changing the colour of the solution which can then be measured. The UV direct assay measures the amount of UV light absorbed by the solution. The documental of this lab is to find concentrations of pro! teins development standards that were made for each assay; hoping to see similar concentrations for all proteins. Methods (Carleton University, 2009) Results In figure 1 you can see the absorbance set across different wavelengths for the p-np solution. The measurable thing to note is at what wavelength the graph peaks at. Table 1: Absorbance and Extinction coefficient values for changing concentrations of...If you want to get a full essay, order it on our website: BestEssayCheap.com

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